This case study examines women’s involvement in peace mediation processes in South Sudan and the barriers they face. Despite the recognized importance of women’s participation, challenges persist in bringing women to the negotiation table and enabling meaningful engagement. Significant barriers continue to impede the achievement of equal representation, influence, and leadership in building and sustaining peace in South Sudan. The case study explores strategies used by women to overcome barriers and be included in discussions and agreements. Moreover, it analyses the legal frameworks utilised by women in South Sudan and assesses the implementation of the R-ARCSS peace agreement. The exclusion of women in the Rome Process is examined, highlighting important contrasts to the inclusive approach of the R-ARCSS. The study’s findings emphasise that the mere presence of women at the mediation table is insufficient since gains can easily be withdrawn without proper implementation. Traditional mediation processes prioritise belligerent parties, hindering the inclusion of women and impeding gender-transformative aspects of peace agreements. Limited political will and support further impede progress. To address these barriers, the study recommends the implementation of additional legal instruments to reduce gender inequities within South Sudan’s Women, Peace, and Security agenda.